Contact an Account ManagerLiquid chromatography method for simultaneous analysis of amino acids and biogenic amines in biological fluids with simultaneous gradient of pH and acetonitrile
Summary
Type: HPLC
Application #: 122400
Detection:
Conditions: Eluent A: 20 mmol/L ammonium acetate – acetonitrile (95:5, v/v) + 1 mmol/L dibutylamine, pH 2.1 (adjusted with 35 % perchloric acid)
Eluent B: 20 mmol/L ammonium acetate – acetonitrile (90:10, v/v) + 5 mmol/L dibutylamine, resulting pH 8.4
Gradient: 74–69 % A in 5 min, 69–63 % A in 10 min, 63–52 % A in 1 min, 52–44 % A in 5 min, 44-32 % A in 4 min, 32–14 % A in 2 min, 14–0 % A in 3 min, 0 % A for 6 min, 0–74 % A in 2 min, 74 % A for 5 min
Flow rate: 1.5 mL/min
Injection volume: 20 µL
Authors: Lozanov, V. et al.
Source: J. Chromatogr. B 860 (2007), 92–97